细菌基因组完成图真菌基因组完成图微生物群落多样性宏基因组测序服务全长转录组测序第三代测序服务非靶向代谢组学靶向代谢组学脂质组学离子组学脂肪酸检测氨基酸检测代谢组学服务定量蛋白质组服务双向电泳服务修饰蛋白质组蛋白质芯片蛋白质谱鉴定蛋白互作检测Western Blot蛋白质组学服务高通量测序数据分析蛋白质组学数据分析代谢组学数据分析芯片数据分析GO/Pathway富集分析相关性和聚类分析共表达网络图靶基因预测调控网络图蛋白相互作用网络图生物信息学服务CRISPR/Cas9载体构建稳定细胞系构建服务模式生物制备服务CRISPR/Cas9技术服务甲基化测序服务甲基化芯片服务甲基化验证服务DNA甲基化服务lncRNA测序服务lncRNA芯片服务lncRNA定量PCR验证lncRNA过表达服务lncRNA抑制服务lncRNA技术服务miRNA测序服务miRNA芯片服务miRNA定量PCR验证miRNA过表达服务miRNA抑制服务miRNA靶基因验证服务miRNA技术服务环状RNA测序服务环状RNA芯片服务环状RNA定量PCR验证环状RNA过表达服务环状RNA抑制服务环状RNA技术服务转录组测序服务表达谱测序服务mRNA/lncRNA/环状RNA 三合一芯片表达谱芯片服务荧光定量PCR验证mRNA过表达服务mRNA抑制服务mRNA技术服务转录因子筛选服务抗体制备服务ChiP-Seq测序服务ChiP-qPCR技术服务EMSA技术服务luciferase 实验服务转录因子技术服务mRNA定量PCR检测miRNA定量PCR检测lncRNA定量PCR检测环状RNA定量PCR检测DNA拷贝数检测相对定量PCR服务绝对定量PCR服务荧光定量PCR服务整体课题服务SNP分型检测服务SSR/STR技术服务病毒包装服务CRISPR/Cas9技术试剂miRNA功能研究试剂lncRNA功能研究试剂环状RNA功能研究试剂mRNA功能研究试剂发表文献样品准备服务流程基金申请

36选7走势图彩票开奖:芯片用户发表《The Plant Journal》

文章附图

体育彩票36选7开奖查询 www.xhkvu.com

  武汉大学生命科学学院王艳萍博士利用言行生物提供的拟南芥芯片服务,发表了《The Plant Journal》杂志,其影响因子为5.972。详情如下:


The Plant Journal  Volume 68, Issue 2, pages 249–261, October 2011

Cytokinin antagonizes ABA suppression to seed germination of Arabidopsis by downregulating ABI5 expression

Yanping Wang1,Lin Li1,Tiantian Ye1,Shujuan Zhao1,Zhao Liu2,Yu-Qi Feng2,Yan Wu1,*

Article first published online: 26 JUL 2011

DOI: 10.1111/j.1365-313X.2011.04683.x

2011 The Authors. The Plant Journal ? 2011 Blackwell Publishing Ltd Issue

Abstract

Abscisic acid (ABA) and cytokinin are key hormones controlling plant development. How ABA and cytokinin interplay to control the transition from a dry seed into a young seedling remains elusive. Here we undertook a gain-of-function genetic screen to identify ABA-insensitive mutants during seed germination in Arabidopsis using an estradiol-inducible approach. In the presence of estradiol, one of these mutants gim1 (germination insensitive to ABA mutant 1) exhibited an elevated level of cytokinin that was attributed to the estradiol-induced expression of AtIPT8 that encodes an isopentenyltransferase for the biosynthesis of cytokinins. Our data on OE-2 and Com-1 transgenic plants carrying the ectopically expressing AtIPT8 gene indicated that the elevation of cytokinin level was responsible for the ABA-insensitivity of gim1 seed germination. Further analyses on alterations of gene transcriptomes in the gim1 mutant demonstrated that the expression of some ABA-inducible genes, including ABI5, was reduced, and could not be restored by exogenous ABA treatment. Moreover, we also failed to observe the ABA-mediated repression of a family of cytokinin signal transducers and transcription repressors called type-A ARR4, ARR5 and ARR6 in the gim1 seedlings. Further analysis demonstrated that type-A ARR4, ARR5 and ARR6 could negatively regulate ABI5 expression, and the physical interaction of ABI5 and type-A ARR4, ARR5 and ARR6 proteins was detected. In summary, our study suggests that the interaction of ABA and cytokinin during seed germination and seedling growth can be mediated by the interplay of transcriptional regulators in Arabidopsis

Supporting Information

Figure S1. Cloning and examining AtIPT8 gene in gim1. (a) The diagram shows XVE-T-DNA insertion site in AtIPT8 gene of gim1 mutant (not in scale). Arrows indicate the transcriptional orientation and T-DNA insertion site in promoter region of AtIPT8 gene. The gray boxes stand for two exons, and a line stands for one intron. (b) The expression level of AtIPT8 gene was examined by semi-quantitative RT-PCR in seedlings of Col and gim1 without (?E.) or with (+E.) induction of estradiol (10 μM). Expression level of ACTIN2 represents the loading control.

Figure S2. Quantifications of relative-contents of cytokinins. Cytokinin contents were quantified with 2-week-old seedlings of Col, gim1, OE-2 and ipt8-1-ko. Seedlings of gim1 and OE-2 were induced with 10 μM estradiol for 24 h. For controls, seedlings of Col and ipt8-1-ko were treated with DMSO. The data represent the means ±SE of two reproducible experiments (FW, fresh weight).

Figure S3. Analysis of gene expression in Col and gim1 plants. The gene expression levels of representative downstream targets in ABA signaling which were identified in our microarray assay were validated by qRT-PCR analysis in Col and in gim1 seedlings. Seedlings were first treated by 10 μM estradiol for 24 h (?ABA) (white bars) and then treated with ABA (50 μM) for 3 h (+ABA) (black bars). The data are the mean values of three reproducible experiments (±SD).

Table S1. Representative ABA- and/or stress-responsive genes identified in microarray analysis were compared in gim1 versus Col.

Table S2. Representative cytokinin-related genes identified in microarray analysis were compared in gim1 versus Col.